Leica DM500, (German) photomicroscope was used to examine and photograph the stained sections. Image analysis and morphometry were performed using the free ImageJ software (Version 1.41; National Institutes of Health, Bethesda, MD, USA). Firstly, the images were calibrated by measuring a known distance on the image then the calibration was globalized to obtain the accurate measurements provided that all the images were taken at the same scale. Proximal convoluted tubule and distal convoluted tubule diameters were measured in Hematoxylin and Eosin-stained sections by drawing a straight line between the outer margins of the tubules using the line Selection tool. As some tubules appeared oval in cross section, two diameters were picked per tubule (length and width) and then the means were calculated. Capillary tuft area in Hematoxylin and Eosin-stained sections was evaluated using the segmented line selection tool. The line was drawn in the capsular space (CS) along the outer margin of the capillary tuft then the area was calculated by the software. Collagen area in Masson’s Trichrome stained sections and the area of expression of anti α- smooth muscle actin was assessed using automatic color selection tool after adjusting the suitable color threshold. The actual area of the tissue was measured by subtracting the empty spaces from the total area of the image. The mean area percentage was calculated according to Shalaby et al. [31 (link)] by dividing the area of the selected color on the actual area of tissue after excluding the background.