Western Blot Analysis of NF-κB and Wnt Signaling

Western blot analysis was realized as previously described [18 (link)]; anti-IκB-α (1:1000, SCB, #sc1643), anti-NF-κB p65 (1:1000; SCB, #sc8414) anti-Wnt3a (Santa Cruz Biotechnology (SCB), Dallas, TX, USA, sc-80457), anti-FZ8 (Bioworld Technology, St. Louis Park, MN, USA). They were also incubated with a β-actin antibody (1:5000; Santa Cruz Biotechnology) for cytosolic proteins or β-catenin antibody (1:5000; Santa Cruz Biotechnology) for nuclear proteins. Protein expression was quantified via densitometry with Bio-Rad ChemiDocTM XRS+ software and normalized with the housekeeping genes β-actin and lamin A/C, as previously reported [39 (link)].