Hematological Analyses and Biomarker Measurement

All hematological analyses were performed by the same cell counter (Coulter HMX, Beckman Coulter, IL Milan, Italy), within 1 h from venipuncture. Soluble P-selectin and von Willebrand factor (VWF) were measured in stored plasma via the Human P-selectin Platinum Enzyme-linked Immunosorbent Assay (ELISA) kit (Affimetrix, eBioscience) [10 (link),21 (link)] and the Hemosil^® von Willebrand Factor Antigen (ELISA kit) (Instrumentation Laboratory), respectively, according to the manufacturer’s instructions. High-sensitivity (hs) C reactive protein (CRP) was measured in serum as described [22 (link)].

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Izzi B., Gialluisi A., Gianfagna F., Orlandi S., De Curtis A., Magnacca S., Costanzo S., Di Castelnuovo A., Donati M.B., de Gaetano G., Hoylaerts M.F., Cerletti C, & Iacoviello L. (2021). Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study. Cells, 10(10), 2737.

Publication 2021

Coulter HMX

Cell Enzyme linked immunosorbent assay High sensitivity c reactive protein Human P selectin Plasma Platinum Serum Venipuncture Von willebrand factor antigen

Corresponding Organization : Istituto Neurologico Mediterraneo

Other organizations : University of Insubria, KU Leuven

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Hematological analyses (including unspecified parameters)
Soluble P-selectin
Von Willebrand factor (VWF)
High-sensitivity (hs) C reactive protein (CRP)

control variables

Cell counter (Coulter HMX, Beckman Coulter, IL Milan, Italy)
Time from venipuncture to analysis (within 1 h)

controls

Positive control: None mentioned
Negative control: None mentioned

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