Mice were treated at 4 and 15 h after tMCAO by subcutaneous (s.c.) injection of 100 µL PBS containing either polyclonal goat anti-mouse osteopontin antibody (R&D Systems, #AF808) or non-immunized goat immunoglobulin G (R&D Systems, #AB-108-C) at a dose of 0.4 mg/kg body weight, as previously described12 (link).
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