U937 cells were induced into activated macrophages with PMA (10 ng/mL) and IL-4 (10 ng/mL) treatment [20 (link)]. Activated macrophages were seeded in the upper chamber with 0.4 μm pore (Corning Life Sciences, Lowell, MA, USA) and were co-cultured with HT-29 and Caco-2 cells in six-well plates at 37 °C and 5% CO2.
Free full text: Click here