Measuring Right Ventricular Hypertrophy

Animals were anesthetized with 3–4% isoflurane and placed on controlled heating pads. Right ventricular systolic pressure (RVSP) was measured by advancing a 2F curve tip pressure transducer catheter (SPR-513, Millar Instruments) into the right ventricle (RV) via the right jugular vein under 1.5–2% isoflurane anesthesia. En-bloc heart and lungs were collected, and lungs were perfused with physiological saline via the right ventricular outflow tract to flush blood cells from the pulmonary circulation. RV hypertrophy was assessed by calculating Fulton’s index, the weight ratio of the RV free wall to the combined left ventricle (LV) + septum [RV/(LV + S)].

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Joshi S.R., Atabay E.K., Liu J., Ding Y., Briscoe S.D., Alexander M.J., Andre P., Kumar R, & Li G. (2023). Sotatercept analog improves cardiopulmonary remodeling and pulmonary hypertension in experimental left heart failure. Frontiers in Cardiovascular Medicine, 10, 1064290.

Publication 2023

SPR-513

Anesthesia Animals Bloc heart Blood cells Catheter Flush Isoflurane Jugular vein Left ventricle Lungs Physiological Pressure transducer Pulmonary circulation Right ventricle Right ventricle hypertrophy Saline Systolic pressure Ventricle septum

Corresponding Organization : Acceleron Pharma (United States)

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Variable analysis

independent variables

Isoflurane anesthesia percentage (3–4% and 1.5–2%)

dependent variables

Right ventricular systolic pressure (RVSP)
Right ventricle (RV) hypertrophy (assessed by Fulton's index)

control variables

Controlled heating pads
Physiological saline perfusion of lungs to flush blood cells from the pulmonary circulation

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