Echocardiography (ECGs) was performed as described previously (25 (link)), using a Vevo® 2100 imaging system (VisualSonics Inc., Toronto, Canada) with the MS250 transducer (13–24 MHz). Animals were anesthetized with 1.5–2% isoflurane and hair was removed from the chest with clippers followed by a depilatory cream. The rats were placed on a warmed platform that recorded ECGs from the paws. ECGs were used to detect bradycardia (heart rate <250 bpm) and arrhythmia. Short axis M-mode recordings at the mid-LV level were used to obtain LV anterior wall (LVAW), posterior wall (LVPW), inner diameter (LVID), ejection fraction (EF), fractional shortening (FS), stroke volume, cardiac output and heart rate.
Protocol detail
Echocardiography Protocol for Rodent Cardiac Assessment
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Animals
Arrhythmia
Axis
Cardiac output
Chest
Echocardiography
Hair
Heart rate
Isoflurane
Rats
Stroke volume
Transducer
Corresponding Organization :
Other organizations : University of Arkansas for Medical Sciences, Moffitt Cancer Center, Central Arkansas Veterans Healthcare System
Top 5 similar protocols
Variable analysis
independent variables
- Anesthesia with 1.5-2% isoflurane
- Bradycardia (heart rate < 250 bpm)
- Arrhythmia
- Left ventricular anterior wall (LVAW)
- Left ventricular posterior wall (LVPW)
- Left ventricular inner diameter (LVID)
- Ejection fraction (EF)
- Fractional shortening (FS)
- Stroke volume
- Cardiac output
- Heart rate
- Use of a Vevo® 2100 imaging system (VisualSonics Inc., Toronto, Canada) with the MS250 transducer (13–24 MHz)
- Hair removal from the chest with clippers followed by a depilatory cream
- Placement of rats on a warmed platform that recorded ECGs from the paws
- None specified
- None specified
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!