Multilineage Differentiation Assay for BM-MSCs

The Mouse Mesenchymal Stem Cell Functional Identification Kit (R&D Systems, Inc. Minneapolis, MN, USA) was used for multipotency evaluation of BM-MSC to differentiate osteoblasts and adipocytes. First, adipocytes were dyed with oil O red to demonstrate lipid droplets. Osteocytes were dyed with Von Kossa to demonstrate bone matrix deposits. BM-MSC culture without differentiation was used as the negative control. Adipocytes and macerated rat bone were positive controls for oil O red and Von Kossa, respectively.

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Quiroz-Reyes A.G., Gonzalez-Villarreal C.A., Limon-Flores A.Y., Delgado-Gonzalez P., Martinez-Rodriguez H.G., Said-Fernandez S.L., Soto-Dominguez A., Rivas-Estilla A.M., Islas J.F., Molina-De la Garza J.F, & Garza-Treviño E.N. (2023). Mesenchymal Stem Cells Genetically Modified by Lentivirus-Express Soluble TRAIL and Interleukin-12 Inhibit Growth and Reduced Metastasis-Relate Changes in Lymphoma Mice Model. Biomedicines, 11(2), 595.

Publication 2023

Mouse Mesenchymal Stem Cell Functional Identification Kit

Adipocytes Bone Bone matrix Lipid droplets Mesenchymal stem cell Mouse Osteoblasts Osteocytes

Corresponding Organization : Universidad Autónoma de Nuevo León

Other organizations : University of Monterrey

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Variable analysis

independent variables

Differentiation of BM-MSC to osteoblasts and adipocytes

dependent variables

Formation of lipid droplets in adipocytes
Formation of bone matrix deposits in osteocytes

control variables

BM-MSC culture without differentiation

positive controls

Adipocytes for oil O red staining
Macerated rat bone for Von Kossa staining

negative control

BM-MSC culture without differentiation

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