Comprehensive Swine Pathogen Detection Protocol

Positive cDNA of JEV and CSFV were from the NJ-2008 strain (GQ918133) and the Shimen strain (AF092448). The positive DNA of PRV was from the SD-2019 strain (GenBank accession No. MW805231). Other positive DNA or cDNA of PCV1, PCV2, PCV3, PCV4, PRRSV, PPV, Chlamydia suis, and Toxoplasma gondii were from specimens previously confirmed using PCR and RT-PCR assays. ASFV-positive DNA was a gift from the Lanzhou Veterinary Research Institute (Lanzhou, China). All the positive nucleic acid above of swine pathogens was stored in the lab for specificity experiments. In all, 383 field specimens, consisting of lymphoid tissues, placentas, sera, and blood, were collected from sows with reproductive issues and aborted piglets in the pig farms of four provinces of East China from 2020 to 2022. The information of the specimens was shown in Table 2.