Methamphetamine hydrochloride (METH, 2.0 mg/kg, i.p., Sigma-Aldrich), mephedrone hydrochloride (racemic mixture; MEPH, 2.0 and 2.5 mg/kg, i.p., Sigma-Aldrich), Nicotine Bitartrate Dihydrate (NIC, 0.5 and 1.0 mg/kg, s.c, Sigma-Aldrich), and Morphine hydrochloride (MOR, 10 mg/kg, i.p., Sigma-Aldrich) were dissolved in sterile 0.9% and administered at the denoted doses. Racemic (+/−) Blebb (Tocris) was diluted to 1 mg/mL in a vehicle of 0.9% SAL and 6.7% DMSO/25% Hydroxypropyl β-Cyclodextrin (HPβCD) and administered at a dose of 10 mg/kg (i.p.) (Young et al. 2016 (link); Briggs et al. 2017 (link); Young et al. 2017 (link)).
Protocol detail
Psychostimulant and Opioid Administration in Rodents
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Cyclodextrin
Dmso
Hydroxypropyl
Meph
Mephedrone
Meth
Methamphetamine hydrochloride
Morphine
Nicotine bitartrate
Sterile
Corresponding Organization :
Other organizations : Scripps Research Institute
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Variable analysis
independent variables
- Methamphetamine hydrochloride (METH, 2.0 mg/kg, i.p., Sigma-Aldrich)
- Mephedrone hydrochloride (racemic mixture; MEPH, 2.0 and 2.5 mg/kg, i.p., Sigma-Aldrich)
- Nicotine Bitartrate Dihydrate (NIC, 0.5 and 1.0 mg/kg, s.c, Sigma-Aldrich)
- Morphine hydrochloride (MOR, 10 mg/kg, i.p., Sigma-Aldrich)
- Racemic (+/−) Blebb (Tocris, 10 mg/kg, i.p.)
- Not explicitly mentioned
- Sterile 0.9% saline as vehicle for METH, MEPH, NIC, and MOR
- Vehicle of 0.9% saline, 6.7% DMSO, and 25% Hydroxypropyl β-Cyclodextrin (HPβCD) for Blebb
- Not explicitly mentioned
- Not explicitly mentioned
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