Tet1/2/3 TKO and wild-type V6.5 mouse ESCs were differentiated to EBs as described before (17 (link)). Day 12 EBs were dissociated into single cells by trypsinization, suspended into PEB buffer, labeled with PE-CD45 (BioLegend, 103106) and FITC-CD41 (Invitrogen, MA5-28367), and subjected to flow cytometry analysis.
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