Genistein and AG1024 Effects on Prostate Cancer Cells

The human PCa cell lines PC3 and DU145 were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Cells were maintained in RPMI-1640 medium (Gibco-BRL; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Gibco-BRL; Thermo Fisher Scientific) at 37°C under 5% CO₂ in a humidified incubator. For cell treatment, PC3 and DU145 cells were cultured in RPMI-1640 medium supplemented with genistein (purity >98%, Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) and AG1024 (Sigma-Aldrich; Merck KGaA) at series concentrations. genistein (0, 10, 20, 30, 50 and 100 µM) and AG1024 (10 µM) (5 (link)) were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich; Merck KGaA). X-ray irradiation was delivered using an X-6 MV photon linear accelerator (Varian Associates Inc., Palo Alto, CA, USA) at room temperature with a dose rate of 2 Gy/min (25 (link)). Cells treated with DMSO were considered as negative control for irradiation or drug treatment.