Inhibitors and Antibodies for Alzheimer's Research

ALLN (Ac-Leu-Leu-Nle-al), Epoxomicin (Epox), CA-074Me, cathepsin G inhibitor I, cathepsin L inhibitor II, synthetic cell-permeable calpastatin peptide, and scrambled peptide were purchased from Millipore. MDL28170, E-64D, cathepsin L inhibitor III, and proteasome substrate Suc-LLVY-AMC were purchased from Enzo life sciences. MG132, cycloheximide, pepstatin A, and L-685,458 were purchased from Sigma-Aldrich. MK-0822 (Odanacatib) was purchased from ChemieTek. The anti-Myc antibody (9B11) was obtained from Cell Signaling. The anti-APP C-terminal antibody C1/6.1 and anti-β-amyloid (1–16) antibody 6E10 were purchased from Covance. The G12A antibody for APP (rabbit polyclonal, clone C7 targeting amino acid residues 732–751 of APP₇₅₁, custom-manufactured by Thermo Fisher Scientific Inc.) has been previously described.^{17 (link)} The anti-Talin antibody (TA205) and protease inhibitor cocktail were purchased from Millipore. The mouse anti-β-actin antibody was purchased from Sigma-Aldrich. The anti-BACE1 antibody (PA1–757) was purchased from Thermo Scientific. The APP₆₉₅-Myc construct in pcDNA3.1 was a generous gift from B. T. Hyman at Harvard University.