Morphological Analysis of CS-SeNPs

For the morphological analysis of CS-SeNPs, a drop of nanoparticle solution was loaded on the carbon-coated copper grid for air-drying, then the samples were observed using transmission electron microscopy (JEM-1200EX, JEOL; Japan) at an accelerating voltage of 80 kV. The surface morphology of Se nanoparticles and elemental composition were studied under Raman Imaging and Scanning Electron Microscopes (RISE-Magna, TESCAN, Czech). The concentration of Se nanoparticles was determined by inductively coupled plasma mass spectrometry (i CAP Q ICP-MS, Thermo Scientific, USA). The size distribution and zeta potential of Se nanoparticles were measured by dynamic light scattering (DLS) using the Zetasizer Nano analyzer (Zetasizer NanoZS, Malvern Panalytical, Malvern, UK) as previously described by Awet et al.27 (link)

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Shao C., Yu Z., Luo T., Zhou B., Song Q., Li Z., Yu X., Jiang S., Zhou Y., Dong W., Zhou X., Wang X, & Song H. (2022). Chitosan-Coated Selenium Nanoparticles Attenuate PRRSV Replication and ROS/JNK-Mediated Apoptosis in vitro. International Journal of Nanomedicine, 17, 3043-3054.

Publication 2022

JEM-1200EX RISE-Magna i CAP Q ICP-MS Zetasizer Nano analyzer

Carbon Copper Mass spectrometry Plasma Scanning electron microscopes Senps Transmission electron microscopy

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Variable analysis

independent variables

Transmission electron microscopy (JEM-1200EX, JEOL; Japan) at an accelerating voltage of 80 kV
Raman Imaging and Scanning Electron Microscopes (RISE-Magna, TESCAN, Czech)
Inductively coupled plasma mass spectrometry (i CAP Q ICP-MS, Thermo Scientific, USA)
Dynamic light scattering (DLS) using the Zetasizer Nano analyzer (Zetasizer NanoZS, Malvern Panalytical, Malvern, UK)

dependent variables

Surface morphology of Se nanoparticles
Elemental composition of Se nanoparticles
Concentration of Se nanoparticles
Size distribution of Se nanoparticles
Zeta potential of Se nanoparticles

control variables

Carbon-coated copper grid for air-drying of nanoparticle solution

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