Hedgehog Pathway Modulation in Cancer

Solasonine was purchased from Yuanye Biotech. (Jinan, China). Prostaglandin E2 (PGE2) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The Hh pathway antagonists GDC-0449 and JQ1 were obtained from Selleckchemicals (Houston, TX, USA). The tumor necrosis factor α (TNF-α), BAY 11-8072 and H89 were purchased from Beyotime (Suzhou, China). Plasmids used in this study were described as previously reported [28 (link)]. The cells used in this study, including light II cells, NIH-3T3 and C3H/10T1/2 mouse embryo fibroblast cells, HEK-293T human epithelial kidney cells, and LS174T colon cancer cells, were all obtained from the American Type Culture Collection (Manassas, VA, USA), and were routinely cultured according to the manufacturer′s instructions. HEK-293T were transfected with ShhN and GFP plasmids. After transfection of 48 h the ShhN conditioned medium (ShhN CM) and GFP CM were collected as previously described [28 (link)].