Hydrogen-Generating Si-Based Diet

AIN-93 M (Oriental Yeast Co., Ltd., Tokyo, Japan) was used as the normal diet. A special diet containing 1.0 wt% Si-based agent in AIN-93 M was made as described previously^{16 (link),20 (link)–22 (link)}. The Si-based agent reacts with water to continuously generate a high amount of hydrogen in the alkaline intestinal environment. The diet also contains pH-adjusting agents, mainly citric acid, which prevent the Si-based agent from reacting with air moisture during feeding.

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Otani N., Tomita K., Kobayashi Y., Kuroda K., Koyama Y., Kobayashi H, & Kubo T. (2022). Hydrogen-generating Si-based agent protects against skin flap ischemia–reperfusion injury in rats. Scientific Reports, 12, 6168.

Publication 2022

AIN-93 M

Agent m Citric acid Diet Hydrogen Intestinal Oriental Yeast

Corresponding Organization :

Other organizations : Osaka University, Osaka Research Institute of Industrial Science and Technology

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Variable analysis

independent variables

Si-based agent concentration (1.0 wt% in AIN-93 M diet)

dependent variables

Not explicitly mentioned

control variables

AIN-93 M diet (normal diet)
PH-adjusting agents (mainly citric acid) to prevent the Si-based agent from reacting with air moisture during feeding

controls

AIN-93 M diet (normal diet) as a positive control
No negative control mentioned

Annotations

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