Protocol detail

Luciferase Assay for BAFF Expression

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The MH7A cells were transfected with pGL3-hBAFF-Luc and pcDNA-lacZ for monitoring transfection efficiency by β-galactosidase assay. Luciferase activity was determined by incubating cell extracts with luciferase substrate (Promega). Luminescence was measured using luminometer (Berthold Technologies, Oak Ridge, TN, USA). Luciferase units of experimental vector were normalized to the control vector in each sample.^{31 (link), 32 (link), 48 (link)}

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Lee J.W., Lee J., Um S.H, & Moon E.Y. (2017). Synovial cell death is regulated by TNF-α-induced expression of B-cell activating factor through an ERK-dependent increase in hypoxia-inducible factor-1α. Cell Death & Disease, 8(4), e2727-.

Publication 2017

luciferase substrate luminometer

Assay Cell Lacz Luciferase Luminescence Pgl3 Promega Transfection Vector β galactosidase

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Variable analysis

independent variables

Transfection of pGL3-hBAFF-Luc and pcDNA-lacZ

dependent variables

Luciferase activity
Luminescence

control variables

Transfection efficiency monitored by β-galactosidase assay

controls

Control vector (used to normalize the luciferase units of the experimental vector)

Annotations

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