Immunofluorescence Analysis of VEEV Capsid Protein

Vero cells grown on coverslips in a 6-well plate were processed for immunofluorescence analysis as previously^{9 (link)}. Antibodies used were anti-VEEV-C protein (BEI Resources, NR-9403) goat primary antibody (1:1000 dilution) and Alexa Fluor 568 donkey anti-goat secondary antibody (1:500 dilution). Slides were imaged using an oil-immersion 60X objective lens on a Nikon Eclipse TE 2000-U confocal microscope. Images were then processed using the Nikon NIS-Elements AR Analysis 3.2 software. Digitized images were analyzed as previously using ImageJ 1.47 public domain software^{9 (link),10 (link)} (see also above).

Free full text: Click here

DeBono A., Thomas D.R., Lundberg L., Pinkham C., Cao Y., Graham J.D., Clarke C.L., Wagstaff K.M., Shechter S., Kehn-Hall K, & Jans D.A. (2019). Novel RU486 (mifepristone) analogues with increased activity against Venezuelan Equine Encephalitis Virus but reduced progesterone receptor antagonistic activity. Scientific Reports, 9, 2634.

Publication 2019

anti-VEEV-C protein NR-9403 Eclipse TE 2000-U confocal microscope NIS-Elements AR Analysis 3.2 software

Alexa 568 Anti antibody Antibodies Antibody C protein Confocal microscope Dilution Donkey Goat Immersion Immunofluorescence Lens Public domain Vero cells

Corresponding Organization :

Other organizations : Monash University, George Mason University, Westmead Institute for Medical Research

Top 5 similar protocols

Variable analysis

independent variables

Antibodies used: anti-VEEV-C protein (BEI Resources, NR-9403) goat primary antibody (1:1000 dilution) and Alexa Fluor 568 donkey anti-goat secondary antibody (1:500 dilution)

dependent variables

Immunofluorescence analysis of VEEV-C protein in Vero cells

control variables

Vero cells grown on coverslips in a 6-well plate
Imaging using an oil-immersion 60X objective lens on a Nikon Eclipse TE 2000-U confocal microscope
Image processing using the Nikon NIS-Elements AR Analysis 3.2 software
Image analysis using ImageJ 1.47 public domain software

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!