Comprehensive Protein Expression Analysis

Western blot analysis was conducted as previously described [24 (link)]. The following primary immunoblotting antibodies were used: anti-GAPDH, anti-E-cadherin, anti-Fibronectin, anti-Vimentin, anti-Snail, and anti-c-Met (Epitomics, Burlingame, CA, USA), anti-β-catenin (Proteintech, Chicago, USA), anti-N-cadherin, anti-c-myc, anti-EGFR, anti-p-EGFR (Tyr1068), anti-GSK3β, anti-p-GSK3β (Ser9), anti-AKT (pan), anti-p-AKT (Ser473), anti-stat3, anti-p-stat3 (Tyr705) and anti-CREB1 (Cell Signaling Technology, Beverly, MA).

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Wang S., Wang X., Li J., Meng S., Liang Z., Xu X., Zhu Y., Li S., Wu J., Xu M., Ji A., Lin Y., Liu B., Zheng X., Xie B, & Xie L. (2017). c-Met, CREB1 and EGFR are involved in miR-493-5p inhibition of EMT via AKT/GSK-3β/Snail signaling in prostate cancer. Oncotarget, 8(47), 82303-82313.

Publication 2017

anti-GAPDH anti-E-cadherin anti-Fibronectin anti-Vimentin anti-Snail anti-c-Met anti-β-catenin anti-N-cadherin anti-c-myc anti-EGFR anti-p-EGFR anti-GSK3β anti-stat3 anti-p-stat3 anti-CREB1

Antibodies Cadherin Egfr Fibronectin Gapdh Gsk3β Snail Stat3 Vimentin Western blot analysis β catenin

Corresponding Organization :

Other organizations : Zhejiang University, Zhejiang Provincial People's Hospital, Tongde Hospital of Zhejiang Province

Top 5 similar protocols

Variable analysis

independent variables

Western blot analysis

dependent variables

GAPDH
E-cadherin
Fibronectin
Vimentin
Snail
C-Met
β-catenin
N-cadherin
C-myc
P-EGFR (Tyr1068)
GSK3β
P-GSK3β (Ser9)
AKT (pan)
P-AKT (Ser473)
Stat3
P-stat3 (Tyr705)
CREB1

control variables

Western blot analysis was conducted as previously described [24 (link)]

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