In situ hybridization was conducted as previously described (Ihermann-Hella et al., 2014 (link)). In brief, an anti-sense RNA probe against Gdnf exons was transcribed and hybridized on thick sections derived from P4 kidneys (10-15 sections/kidney, n=3 kidneys/genotype) embedded in 4% low melting agarose (NuSieve GTG, Lonza). BM Purple was used for colorimetric reaction.
Free full text: Click here