Drosophila Rearing and Genetic Lines

Flies were raised on standard cornmeal food media at 25°C and 70% relative humidity under a 12:12-hour light: dark cycle. The “Cantonized” w¹¹¹⁸w (CS10) strain was used as a wild-type control. The MB-GAL80, UAS-GCaMP6m, and OK107-GAL4 flies were obtained from Bloomington Drosophila stock center. The UAS-eNpHR-YFP; UAS-eNpHR-YFP fly line was obtained from Ann-Shyn Chiang. The RNAi lines were obtained from the Vienna Drosophila RNAi Center or TRiP RNAi fly stocks. All RNAi lines from the Vienna Drosophila RNAi Center have been described previously[12 (link)]. The VT30604-GAL4, VT44966-GAL4, C739-GAL4, VT49246-GAL4, VT0765-GAL4, UAS-shi^ts, and tubP-GAL80^ts flies have been described[20 (link)].

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Shyu W.H., Lee W.P., Chiang M.H., Chang C.C., Fu T.F., Chiang H.C., Wu T, & Wu C.L. (2019). Electrical synapses between mushroom body neurons are critical for consolidated memory retrieval in Drosophila. PLoS Genetics, 15(5), e1008153.

Publication 2019

UAS-GCaMP6m OK107-GAL4

Center lines Drosophila Flies Food Humidity Light Rnai Strain

Corresponding Organization : Chang Gung Memorial Hospital

Other organizations : National Chi Nan University, National Cheng Kung University

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Variable analysis

independent variables

Genetic manipulations (e.g., RNAi lines, UAS-eNpHR-YFP, etc.)

dependent variables

Not explicitly mentioned

control variables

Fly strain (Cantonized w1118 w (CS10) as a wild-type control)
Temperature (25°C)
Relative humidity (70%)
Light/dark cycle (12:12-hour)

controls

Positive control: Cantonized w1118 w (CS10) strain as a wild-type control
No negative controls specified

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