Dietary Composition Impacts on Rodent Metabolism

A semi-synthetic diet containing 5% sucrose polybehenate, a non-absorbable food additive, was prepared by Research Diets, Inc. (New Brunswick, NJ, USA), as we reported previously [10 (link)]. Additionally, two pelleted semi-purified, nutritionally complete experimental diets (AIN-93M [11 (link)]) were purchased from Research Diets, Inc., New Brunswick, NJ, USA. The high-fat diet (HFD, catalog #: D03082706) contained 20% fat (19 g of butter oil and 1 g of soybean oil in 100 g of diet to provide essential fatty acids), and the low-fat diet (LFD, catalog #: D03082705) contained 3 g of butter oil and 1 g of soybean oil in 100 g of diet [12 (link)]. We equalized the amount of protein and all essential minerals and vitamins required for rodents [8 (link)] per kJ for both HFD and LFD [12 (link)]. Diets given to the GF mice were sterilized with double gamma irradiation, and the diets given to CV mice were regularly irradiated.