Pharmacological Modulation of Murine Metabolism

Six-weeks old BALB/c female mice (Taconic) were treated with MS023 (80 mg/kg body weight, by i.p. injection), GSK3368715 (80 mg/kg body weight, by gastric gavage), or respective vehicle, one dose daily for 21 days. MS023 (SelleckChem, #S8112) was dissolved in NMP (Sigma, #328643), and then sequentially diluted with 20% Captisol (SelleckChem, #S4592), PEG-400 (Sigma, PX1286B-2) and saline, with a final ratio of 5:20:20:55 (NMP/20% Captisol/PEG-400/saline, v/v/v/v)^{65 (link)}. GSK3368715 (SelleckChem, #S8858) was dissolved in ddH₂O. Then, tissues and organs were collected and analyzed by immunoblotting.

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Liu J., Bu X., Chu C., Dai X., Asara J.M., Sicinski P., Freeman G.J, & Wei W. (2023). PRMT1 mediated methylation of cGAS suppresses anti-tumor immunity. Nature Communications, 14, 2806.

Publication 2023

BALB/c female mice MS023 NMP Captisol PEG-400 GSK3368715

Balb c mice Body weight Captisol Female Gastric Gavage Peg 400 Saline Tissues

Corresponding Organization :

Other organizations : Harvard University, Beth Israel Deaconess Medical Center, Dana-Farber Cancer Institute

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Variable analysis

independent variables

MS023 (80 mg/kg body weight, by i.p. injection)
GSK3368715 (80 mg/kg body weight, by gastric gavage)

dependent variables

Tissues and organs collected and analyzed by immunoblotting

control variables

Six-weeks old BALB/c female mice (Taconic)
Respective vehicle treatments

controls

Positive control: Not explicitly mentioned.
Negative control: Respective vehicle treatments

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