Mitochondrial Function Assay Protocol

The mitochondrial membrane potential (MMP) was measured using MitoProbe™ JC-1 Assay Kit (#M34152, Thermo Fisher Scientific, Waltham, MA) as previously described [15 (link)]. The activities of complexes on the electron transport chain were measured using Complex I Enzyme Activity Microplate Assay Kit (Abcam, #ab109721); Complex II Enzyme Activity Microplate Assay Kit (Abcam, #ab109908); and Complex IV Human Enzyme Activity Microplate Assay Kit (Abcam, #ab109909) according to the manufacturers’ protocol. Results were normalized by the protein amounts in each assay.

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Guo N.L., zhang J.X., Wu J.P, & Xu Y.H. (2017). Isoflurane promotes glucose metabolism through up-regulation of miR-21 and suppresses mitochondrial oxidative phosphorylation in ovarian cancer cells. Bioscience Reports, 37(6), BSR20170818.

Publication 2017

MitoProbe™ JC-1 Assay Kit Complex I Enzyme Activity Microplate Assay Kit Complex II Enzyme Activity Microplate Assay Kit Complex IV Human Enzyme Activity Microplate Assay Kit

Assay Complex i Complex ii Complex iv Electron transport Enzyme assay Human Mitochondrial membrane potential Protein

Corresponding Organization :

Other organizations : Huadong Hospital, Fudan University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Mitochondrial membrane potential (MMP) measured using MitoProbe™ JC-1 Assay Kit
Activities of complexes on the electron transport chain measured using:
- Complex I Enzyme Activity Microplate Assay Kit
- Complex II Enzyme Activity Microplate Assay Kit
- Complex IV Human Enzyme Activity Microplate Assay Kit

control variables

Protein amounts in each assay, used for normalization

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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