Cryogenic Homogenization of Plant Samples

The conditions for the homogenization of samples were established following the previously reported methods, with some modifications [76 (link),77 (link)]. An MB2000 Multi-bead Shocker (Yasui Kikai Co., Ltd., Osaka, Japan) was used to cryohomogenize the rhizome and leaf samples into fine powder by running into 2 circles at 2800 rpm for 16 s per round. Each fine powder sample, weighing 1.0 g, was placed into a 20 mL headspace screw-top clear GC glass vial (Agilent Technologies Co., Ltd., Waldbronn, Germany). The sample powders were very susceptible to melting, especially the leaf powder. Therefore, as soon as the samples were weighed, GC vials were kept on ice until the all of the chemicals had been added.

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Thawtar M.S., Kusano M., Yingtao L., W.u.n.n.a., Thein M.S., Tanaka K., Rivera M., Shi M, & Watanabe K.N. (2023). Exploring Volatile Organic Compounds in Rhizomes and Leaves of Kaempferia parviflora Wall. Ex Baker Using HS-SPME and GC–TOF/MS Combined with Multivariate Analysis. Metabolites, 13(5), 651.

Publication 2023

MB2000 Multi-bead Shocker

Leaf Powder Rhizome

Corresponding Organization : University of Tsukuba

Other organizations : Yezin Agricultural University, Tokyo University of Agriculture, Tokyo University of Information Sciences, University of the Philippines Los Baños

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Variable analysis

independent variables

Homogenization method (MB2000 Multi-bead Shocker at 2800 rpm for 16 s per round)

dependent variables

Powder sample characteristics (e.g., fine powder, susceptibility to melting)

control variables

Sample weight (1.0 g)
Storage conditions (kept on ice)

controls

No positive or negative controls were explicitly mentioned.

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