Rainbow trout RBCs were obtained from the peripheral blood of fish which died through overexposure to tricaine (tricaine methanesulfonate, Sigma-Aldrich, Madrid, Spain; 0.2 g/L), as previously described [8 (link)]. Briefly, peripheral blood was sampled from the caudal vein. Then, RBCs were purified by two consecutive density gradient centrifugations (7206 g, Ficoll 1.007; Sigma-Aldrich). Purity of RBCs of 99.9% was estimated by optical microscopy evaluation (Figure S1 ).
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