Antioxidant Enzyme Activity in Soybean Hairy Roots

The activities of POD and SOD, H₂O₂ content, and GSH level in soybean hairy roots were measured as described previously [63 ,64 (link)]. In brief, total proteins were extracted using the vegetable protein extraction kit (KeyGene BioTECH, Nanjing, China) and quantified using a BCA protein assay kit (KeyGene BioTECH). The activities of POD and SOD, H₂O₂ content, and GSH level were measured using a peroxidase kit (Jiancheng BioTECH, Nanjing, China), superoxide dismutase kit (Keming BioTECH, Suzhou, China), H₂O₂ test kit (Jiancheng BioTECH, Nanjing, China), and GSH test kit (Jiancheng BioTECH, Nanjing, China) according to the instructions from the manufacturers and previously published methods [63 ,64 (link)]. All measurements were performed with four biological replications and three independent soybean hairy roots were used in each replication.