Confocal Imaging of In Vivo Neuronal Morphology

Confocal imaging of in vivo neuronal morphology was performed as previously described (Das et al., 2017 (link); Turner et al., 2016 (link)). Briefly, third instar larvae were mounted on slides with 1:5 (v/v) diethyl ether:halocarbon oil and imaged on a Zeiss LSM780 confocal system. Z-stacks of class IV md neurons were obtained and neuromorphometric analyses of two-dimensional maximum projections of the z-stacks were performed using Adobe Photoshop and ImageJ (Analyze Skeleton plug in: ImageJ.net/AnalyzeSkeleton">http://ImageJ.net/AnalyzeSkeleton) as previously described, with modification (Iyer et al., 2013 (link)). Statistical significance was tested using one-way ANOVA with Bonferroni multiple comparison post hoc test in GraphPad Prism.