As described previously11 (link). WB analysis was performed by using the whole-cell lysates (30 μg per well) of the colonic mucosal stripping of TgM9 and their WT littermates’ mice with and without CAC. The primary antibodies used were anti-MMP9 (Cell Signaling, Beverly, MA, USA), anti-γH2AX (Abcam), anti-MDC1 (Novus Biologicals, Littleton, CO), anti-MLH1 (Abcam), anti-MSH2 (Abcam), and anti-PCNA (Abcam). Goat anti-mouse secondary antibody (Bio-Rad, Hercules, CA) or goat anti-rabbit secondary antibody (Abcam) were used. Anti-GAPDH (Abcam) or anti-β actin (Sigma) antibodies were used as the WB loading controls. Bio-Rad Quantity One software was used for densitometry analysis.
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