Adipogenesis Modulation by PIEZO1 Agonist

Preadipocytes were seeded in six well-plates and induced to differentiate into adipocytes in standard adipogenic media alone, as previously described by our group (31 (link)), in the presence of the PIEZO1 agonist Yoda1 10 µM (Tocris, Cat N° 5586), a concentration reported in different cell types (18 (link), 29 (link), 33 (link)–36 (link)). Adipogenesis was evaluated using Bodipy 493/503 (ThermoFisher Cat N° D3922), a neutral lipid staining, and the nuclear stain NucSpot^® Live 650 (Biotium, Cat N° 40082) and reported as Bodipy fluorescence intensity/nuclei count using long-term live-cell imaging IncuCyte^® S3 system. Cultured cells were imaged every 6 hours during 4 days in culture. Quantification of cell images after 4 days was performed using IncuCyte ZOOM™ software.