Transcriptional profiling was done using the Illumina HumanHT-12 v4 array (Illumina, San Diego, CA) and the data was processed as previously described (26 (link)). Genes were considered aberrantly expressed if the fold change between samples and controls exceeded 1.5-fold and expression differences common to all cell lines were included in subsequent analyses. A network consisting of human protein-protein interaction pairs was generated using MIMI Plugin in Cytoscape. Protein clusters representing highly interconnected regions in the network were generated using the MCODE plugin in Cytoscape.
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Transcriptional Profiling and Protein Network Analysis
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Corresponding Organization :
Other organizations : University Health Network, Princess Margaret Cancer Centre, University of British Columbia, University of Toronto
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Variable analysis
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- Transcriptional profiling was done using the Illumina HumanHT-12 v4 array
- Genes were considered aberrantly expressed if the fold change between samples and controls exceeded 1.5-fold
- Expression differences common to all cell lines were included in subsequent analyses
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