Transmission Electron Microscopy of oEVs

Aliquots of oEVs samples (one per stage and per side) were fixed with glutaraldehyde 2% for observation under TEM, as previously described [9 (link)]. The micrographs were obtained using TEM HITACHI HT 7700 Elexience at 80 kV (with a charge-coupled device camera AMT) and JEM 1011 (JEOL, Tokyo, Japan) equipped with a Gatan digital camera driven by Digital Micrograph software (Gatan, Pleasanton, CA, USA) at 100 kV. The processing of the photos and vesicle size calculation (min 300 vesicles per stage) were carried out by ImageJ software.

Free full text: Click here

Gatien J., Mermillod P., Tsikis G., Bernardi O., Janati Idrissi S., Uzbekov R., Le Bourhis D., Salvetti P., Almiñana C, & Saint-Dizier M. (2019). Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle. International Journal of Molecular Sciences, 20(24), 6339.

Publication 2019

HT 7700 Elexience JEM 1011 Digital Micrograph software

Device Glutaraldehyde

Corresponding Organization :

Other organizations : Physiologie de la Reproduction et des Comportements, Université de Tours, Bioengineering Center

Top 5 similar protocols

Variable analysis

independent variables

Aliquots of oEVs samples (one per stage and per side)

dependent variables

Morphology and size of extracellular vesicles (EVs)

control variables

Glutaraldehyde 2% fixation for TEM observation
Microscope settings (HITACHI HT 7700 Elexience at 80 kV, JEM 1011 at 100 kV)
Image processing and vesicle size calculation using ImageJ software

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!