Murine Melanoma Cell Viability Assay

Murine melanoma B16F10 cells were purchased from the Korean Cell Line Bank (KCLB, Seoul, Korea) and cultured in DMEM supplemented with 10% FBS and 1% streptomycin, and then incubated at 37 °C, humidified with 5% atmospheric CO₂. Cell viability analyses were performed as previously described [42] (link). Briefly, cells seeded at a density of 1 × 10⁴ cells/well in a 96-well plate for 24 h. On the following day, the cells were exposed to different concentrations of BID3 and incubated for 24 or 48 h, respectively. Then, 10 µL EZ-Cytox solution was added to each well and the cells were incubated for 2–4 h. Absorbance was determined using ELISA at a wavelength of 450 nm. Each assay was performed in triplicate.