Quantitative Analysis of Inflammatory Markers in BV-2 Cells

Total RNA was extracted from the BV-2 cells of different treatment groups using the EZ-Press RNA purification kit (EZ Bioscience, Roseville, CA, USA). Then, a Reverse Transcription Kit (EZ Bioscience, Roseville, CA, USA) was used to convert the extracted RNA into cDNA using specific primers (EZ Bioscience, Roseville, CA, USA) to amplify the cDNA templates. The mRNA levels of various genes (COX2, TNF-α, IL-1β, and IL-6) compared with GAPDH were evaluated by 2^−ΔΔCt. The primer sequences used in this study refer to previous studies [52 (link),53 (link),54 (link)] (Table 1).

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Wei X., Wang D., Liu J., Zhu Q., Xu Z., Niu J, & Xu W. (2024). Interpreting the Mechanism of Active Ingredients in Polygonati Rhizoma in Treating Depression by Combining Systemic Pharmacology and In Vitro Experiments. Nutrients, 16(8), 1167.

Publication 2024

EZ-Press RNA purification kit Reverse Transcription Kit

Corresponding Organization : University of Science and Technology of China

Other organizations : Hefei University of Technology

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Variable analysis

independent variables

Different treatment groups

dependent variables

MRNA levels of COX2, TNF-α, IL-1β, and IL-6

control variables

GAPDH

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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