The blood samples were collected after 0.5, 1, 2, 3, 6, 8, 12, 24, 36, and 48 h of dosing. The collected samples were centrifugated at 3500 rpm for 10 min to obtain the supernatant and stored at −80 °C for further analysis.
The plasma concentration of abemaciclib was analyzed by the LC-MS/MS method with the Agilent 1290 series liquid chromatography system and the Agilent 6460 triple-quadruple mass spectrometer (Agilent Technologies, USA). The reaction conditions were conducted according to previous reports (Naz et al. 2018 (link)). Briefly, the samples were separated on the C18 column with the mobile phase (0.1% formic acid in water: acetonitrile). The temperature of the column was 25 °C with a flowing rate of 0.4 mL/min and an injection volume of 5 μL. The MRM mode was conducted with the collision energy of 30 eV. The MS/MS conditions were as follows: fragmentor, 110 V; capillary voltage, 3.5 kV; nozzle voltage, 500 V; nebulizer gas pressure (N2), 40 psig; drying gas flow (N2), 10 L/min; gas temperature, 350 °C; sheath gas temperature, 400 °C; sheath gas flow, 11 L/min.
The pharmacokinetics of abemaciclib was evaluated with corresponding parameters, including the area under the curve (AUC), half-life (t1/2), the maximum concentration (Cmax), the time reached Cmax (Tmax), and the clearance rate (ClzF).
The plasma concentration of abemaciclib was analyzed by the LC-MS/MS method with the Agilent 1290 series liquid chromatography system and the Agilent 6460 triple-quadruple mass spectrometer (Agilent Technologies, USA). The reaction conditions were conducted according to previous reports (Naz et al. 2018 (link)). Briefly, the samples were separated on the C18 column with the mobile phase (0.1% formic acid in water: acetonitrile). The temperature of the column was 25 °C with a flowing rate of 0.4 mL/min and an injection volume of 5 μL. The MRM mode was conducted with the collision energy of 30 eV. The MS/MS conditions were as follows: fragmentor, 110 V; capillary voltage, 3.5 kV; nozzle voltage, 500 V; nebulizer gas pressure (N2), 40 psig; drying gas flow (N2), 10 L/min; gas temperature, 350 °C; sheath gas temperature, 400 °C; sheath gas flow, 11 L/min.
The pharmacokinetics of abemaciclib was evaluated with corresponding parameters, including the area under the curve (AUC), half-life (t1/2), the maximum concentration (Cmax), the time reached Cmax (Tmax), and the clearance rate (ClzF).
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