HCT-8 cells (ATCC, cat#CCL-244) were cultured in RPMI (Gibco, Waltham, MA, USA, cat#22400089) supplemented with 10% horse serum (Gibco, cat#26050-088) and 100 U/mL penicillin plus 100 μg/mL streptomycin (Gibco, cat#15140122). We cultured 293 F cells (Thermo Fisher, Waltham, MA, USA, cat#R79007) in Freestyle 293 media (Thermo Fisher, cat#12338026). We cultured 3T3 CD40L/IL-2/IL-21 feeder cells in DMEM supplemented with 10% fetal calf serum (Peak, Wellington, CO, USA, cat#PS-FB2), penicillin and streptomycin, plus 0.4 mg/mL geneticin as described [19 (link)]. Irradiation was performed with 5000 rads.
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