Lyophilized shoots of D. forrestii (100 mg amounts) were extracted with 80% methanol by sonification (UD-20 ultrasonic disintegrator). The extraction is described in more detail by Weremczuk-Jeżyna et al. [18 (link)].
The plant material was subjected to quantitative HPLC analysis as described by Weremczuk-Jeżyna et al. [18 (link)]. The HPLC system consisted of a diode array detector (Waters 2998), a binary HPLC pump (Waters 2545), and an autosampler (Waters 2767). Separations were carried out on an XBridge C18 OBD column. The mobile phase (A) consisted of 0.1% trifluoroacetic acid in the water, with mobile phase B consisting of 0.1% trifluoroacetic acid in acetonitrile. The flow rate was 1.6 mL/min. UV spectra were recorded at 190–700 nm and chromatograms were acquired at 325 nm. The phenolic compounds were identified by comparing their UV spectra and retention times with those of reference compounds and/or literature data [18 (link)]. Phenolic standards such as rosmarinic acid, caffeic acid, and chlorogenic acid were purchased from Sigma-Aldrich (Darmstadt, Germany), and salvianolic acid B was purchased from Extrasynthese (Genay, France). Compounds for which pure standards were not available were quantified according to the calibration curve of similar standards.
The plant material was subjected to quantitative HPLC analysis as described by Weremczuk-Jeżyna et al. [18 (link)]. The HPLC system consisted of a diode array detector (Waters 2998), a binary HPLC pump (Waters 2545), and an autosampler (Waters 2767). Separations were carried out on an XBridge C18 OBD column. The mobile phase (A) consisted of 0.1% trifluoroacetic acid in the water, with mobile phase B consisting of 0.1% trifluoroacetic acid in acetonitrile. The flow rate was 1.6 mL/min. UV spectra were recorded at 190–700 nm and chromatograms were acquired at 325 nm. The phenolic compounds were identified by comparing their UV spectra and retention times with those of reference compounds and/or literature data [18 (link)]. Phenolic standards such as rosmarinic acid, caffeic acid, and chlorogenic acid were purchased from Sigma-Aldrich (Darmstadt, Germany), and salvianolic acid B was purchased from Extrasynthese (Genay, France). Compounds for which pure standards were not available were quantified according to the calibration curve of similar standards.
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