Zebrafish Protein Extraction and Immunoblotting

Protein extraction of embryonic zebrafish and immunoblotting was carried out as described [16 (link),17 (link)]. For immunoblotting following primary antibodies were used: rabbit anti-Hdac1 (1:500, Abcam #ab33278), rabbit anti-acetyl-Histone H3 (1:1000, Millipore #06–599) rabbit anti-acetyl Histone H4 (1:1000, Abcam #ab177790). For loading control rabbit anti-Histone H3 (1:2500,Sigma/ #H0164), rabbit anti-Pan Cadherin (1:10000, Abcam #ab16505), or rabbit anti-LaminB1 (1:1000; Abcam #16048) were used. Signals were detected by chemiluminescence (anti-mouse IgG HRP-linked, anti-rabbit IgG HRP-linked, Cell Signaling #7076/#7074) using a Luminescent image analyzer (Image Quant LAS4000mini, D-79111 Freiburg, Germany).

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Bühler A., Gahr B.M., Park D.D., Bertozzi A., Boos A., Dalvoy M., Pott A., Oswald F., Kovall R.A., Kühn B., Weidinger G., Rottbauer W, & Just S. (2021). Histone deacetylase 1 controls cardiomyocyte proliferation during embryonic heart development and cardiac regeneration in zebrafish. PLoS Genetics, 17(11), e1009890.

Publication 2021

ab33278 ab177790 H0164 ab16505 anti-mouse IgG HRP-linked, Image Quant LAS4000mini

Anti igg Antibodies Cadherin 1 Chemiluminescence Embryonic Histone h3 Histone h4 Igg hrp Luminescent Mouse Protein Rabbit Zebrafish

Corresponding Organization : University of Pittsburgh

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Variable analysis

independent variables

Protein extraction of embryonic zebrafish

dependent variables

Hdac1 protein expression
Acetyl-Histone H3 protein expression
Acetyl-Histone H4 protein expression

control variables

Histone H3 protein expression
Pan Cadherin protein expression
LaminB1 protein expression

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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