Prior to transplantation, MSC1 and MSC1-HI cells were cultured and aggregated in non-tissue culture plates using Ham’s F10 media containing supplements for 48 h at 37 °C [17 (link)]. Six million MSC1 or MSC1-HI cells (estimated using a DNA assay [54 (link)]) were transplanted under the left renal subcapsular space of male BALB/c mice rendered diabetic by streptozotocin (270–275 mg/kg of body weight, Sigma Chemical Co., Burlington, MA, USA) injected intraperitoneally 7 days before transplantation, as previously described in Kaur et al., 2014 [16 (link)].
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