Optogenetic Stimulation Protocols for Pain Assays

Prior to behavioral tests, and 15 min after bilateral intracranial administrations, optic fibers were connected to a 473 nm laser diode with an FC/PC adapter (Shanghai Dream Lasers, China). And the laser intensity was measured with an instrument of power meter (Thorlabs, Newton, NJ, USA). The output of laser was delivered using a TTL pulse-generating box (Tucker-Davis Technologies, USA), and then was split evenly to two fibers (for bilateral stimulation) with a splitter. Before behavioral tests, laser output in each terminal of the two fibers was verified with an instrument of power meter to ensure that equal power was provided. As previously described [6 (link), 7 (link)], a laser protocol that included alternating light-on and light-off epochs for 30 s each was provided for the duration of Hargreaves test, mechanical allodynia test, cold allodynia test, and CPP test. Light was delivered at 20 Hz with 10 ms pulse length within the light-on epoch.

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Zeng F., Zhang Q., Liu Y., Sun G., Li A., Talay R.S, & Wang J. (2021). AMPAkines potentiate the corticostriatal pathway to reduce acute and chronic pain. Molecular Brain, 14, 45.

Publication 2021

power meter TTL pulse-generating box

Allodynia Behavioral tests Cold Dream Epoch Laser diode Light Pulse

Corresponding Organization :

Other organizations : Nanchang University, First Affiliated Hospital of Nanchang University, New York University

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Variable analysis

independent variables

Bilateral intracranial administrations

dependent variables

Behavioral tests (Hargreaves test, mechanical allodynia test, cold allodynia test, and CPP test)

control variables

Laser intensity measured with a power meter
Equal power provided to the two fibers for bilateral stimulation
Laser protocol with alternating light-on and light-off epochs for 30 s each, delivered at 20 Hz with 10 ms pulse length during light-on epochs

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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