Signaling Pathways in Chronic Myelogenous Leukemia

K562 CD34⁺ cells and primary CML CD34⁺ cells(2.5 × 10⁵ cells/well in 6-well plates) were exposed to various stimulating conditions for the indicated times. The lysated preparations were carried out by Western blot as previously mentioned. Antibodies for Western blot were used in our research: p-JAK2^Tyr1007/1008, p-STAT5^Tyr694, JAK2, STAT5, BCR-ABL, CrkL, Survivin, Bcl-2, Mcl-1, XIAP, cleaved-caspase 3, bax, caspase 3, GAPDH, DNMT1 were obtained from Cell Signaling Technology (Danvers, MA, USA). SHP-1 and cyclin D2 were ordered from Abcam(Cambridge, MA, USA).

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Xu X., Ji S., Chen Y., Xia S., Li Y., Chen L., Li Y., Zhang F., Zhang Z, & Zheng S. (2023). Induction of DNMT1-dependent demethylation of SHP-1 by the natural flavonoid compound Baicalein overcame Imatinib-resistance in CML CD34+ cells. Cell Communication and Signaling : CCS, 21, 47.

Publication 2023

p-JAK2Tyr1007/1008 p-STAT5Tyr694 STAT5 BCR-ABL Survivin Bcl-2 Mcl-1 cleaved-caspase 3 bax caspase 3 GAPDH DNMT1 SHP-1 cyclin D2

Antibodies Bcl 2 Caspase 3 Cells Crkl Cyclin d2 Dnmt1 Gapdh Jak2 K562 cells Stat5 Survivin Western blot

Corresponding Organization : Nanjing University of Chinese Medicine

Top 5 similar protocols

Variable analysis

independent variables

Various stimulating conditions

dependent variables

P-JAK2^Tyr1007/1008
P-STAT5^Tyr694
STAT5
BCR-ABL
Survivin
Bcl-2
Mcl-1
Cleaved-caspase 3
Caspase 3
DNMT1
SHP-1
Cyclin D2

control variables

K562 CD34+ cells
Primary CML CD34+ cells (2.5 × 10^5 cells/well in 6-well plates)
GAPDH

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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