ATAC-seq Library Generation Protocol

ATAC-seq libraries for zinc-induced and non-induced libraries were generated following the protocol outlined previously^{41 (link)}. Briefly, 5 × 10⁵ cells were re-suspended in ATAC-Resuspension buffer (0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin). Isolated nuclei were then incubated in a transposition mixture containing 100 nM final transposase at 37 °C for 30 min. DNA was extracted and purified using the Zymo DNA Clean and Concentrator-5 Kit. Libraries were pre-amplified with five cycles using NEBNext Master Mix, additional cycles were determined by SYBR Green qPCR. DNA was purified (Zymo) after amplification and quantitated using the NEBNext quant kit by qPCR. Paired-end libraries were sequenced on the Illumina HiSeq 2000 platform.