Effect of Integrin Expression on TGF-β Signaling in MECs

The effect of WT and D119A β₃ integrin expression on various TGF-β stimulated activities in MECs was determined as follows: cell proliferation using 10,000 cells/well in a [³H]thymidine incorporation assay, as described elsewhere [35 (link)]; cell invasion induced by 10% serum using 350,000 cells/well in a modified Boyden-chamber coated with Matrigel matrices (diluted 1:25 in serum-free Dulbecco's modified Eagle's medium), as described elsewhere [35 (link),40 (link)]; and gene expression using 30,000 cells/well in a synthetic pSBE-luciferase reporter gene assay, as described previously [35 (link)].