Both types of extracts prepared were subjected to chromatographic analysis by conducting High-Performance Liquid Chromatography with Diode Array Detector (HPLC-DAD). The amount of 1 mg/mL of each extract was analyzed in a VWR-Hitachi Elite LaChrom®® equipped with a LiChroCART®®RP-18, 5 μm, 250 × 4 mm, 100 Å column from Merck, autosampler L-2200, column oven L-2300 and diode array detector (DAD) L-2455. In this step, the mobile phase consisted of 0.05% (v/v) of trifluoracetic acid (Merck) in water and acetonitrile (Carlo Erba, Val-de-Reuil, France). Detection was carried out between 200 and 600 nm using diode array detector (DAD), and data acquisition was carried out by using EZChrom Elite®® Hitachi Japan software.
LC/HRMS analysis was performed by liquid chromatography-high resolution tandem mass spectrometry (LC-HRMS/MS), as described in [26 (link),27 (link)]. Acquired data were processed by DataAnalysis 4.1 software (Bruker Daltonik GmbH, Bremen, Germany). This identification was carried out by comparing the retention time as well as exact mass from the DataAnalysis®® program version 4.4 from BRUKER, with results in [26 (link)].
LC/HRMS analysis was performed by liquid chromatography-high resolution tandem mass spectrometry (LC-HRMS/MS), as described in [26 (link),27 (link)]. Acquired data were processed by DataAnalysis 4.1 software (Bruker Daltonik GmbH, Bremen, Germany). This identification was carried out by comparing the retention time as well as exact mass from the DataAnalysis®® program version 4.4 from BRUKER, with results in [26 (link)].
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