Serum Lipid Profile Analysis Protocol

Blood analysis was performed twice: once before (0 weeks) and once after the end of the study (8 weeks). After an overnight fast of at least 8 h, blood samples of 5 mL were collected and coagulated for at least 30 min at room temperature before being centrifuged at 3500 rpm for 7 min. For each sample, 1 mL of separated serum was placed in a microtube and frozen. The frozen serum was analyzed by the Buraydah Central Hospital Laboratories using a Chemistry Analyzer (Beckman Coulter UniCel DxC 600; Beckman Coulter, Brea, CA, USA). In accordance with Choi et al. [25 (link)], the analysis included the total cholesterol, triglyceride, LDL-C, and HDL-C levels.