Cytokine Measurement Protocols

IL-10, CXCL10 and IL-27 production were measured using an IL-10 ELISA kit from BD, a CXCL10 ELISA kit from R&D, or anti-mouse IL-27p28 and biotin-conjugated anti-mouse IL-27p28 antibodies from R&D (Minneapolis, MN, USA) as described in [25 (link)]. Alternatively, IL-27 was also measured by mouse IL-27 ELISA Ready-Set-Go! Kit from eBioscience, following manufacturer’s instructions.

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Lee M.H., Gallo P.M., Hooper K.M., Corradetti C., Ganea D., Caricchio R, & Gallucci S. (2019). The cytokine network Type I Interferon, IL-27 and IL-10 is augmented in murine and human lupus.. Journal of leukocyte biology, 106(4), 967-975.

Publication 2019

IL-10 ELISA kit CXCL10 ELISA kit

Anti d antibodies Biotin Elisa Il 10 Il 27 Mouse

Corresponding Organization : Temple University

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

IL-10 production
CXCL10 production
IL-27 production

control variables

Not explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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