Expression Analysis of Cotton GW2-like Genes

Wild-type cotton (TM-1) and Arabidopsis (Col-0) were planted in the experimental field and greenhouse of the Institute of Cotton Research of Chinese Academy of Agricultural Sciences under conventional field management conditions in Anyang, China. To detect the relative expression of GW2-like genes, different samples were collected from WT cotton at different stages of ovule development. All ovule samples were self-pollinated. Total RNA was isolated from samples using Fast Pure Plant Total RNA Isolation Kit (Vazyme, Nanjing, China). The qRT-PCR analysis primers were listed in Supplementary Table 1. Gh_D03G0370 (GhActin3) (Li et al., 2005 (link)) and AT3G18780 (AtActin2) (Wang et al., 2016 (link)) were used as internal controls for the qPCR experiments.