Chromatin immunoprecipitation (ChIP), quantitative RT-PCR, and ChIP-qPCR were performed as described earlier (13 (link),29 (link),30 (link)). Briefly, cells were either transfected, infected or treated with various inhibitors (R)-9b; CPTH2 (Sigma, Cat#C9873)], fixed in formaldehyde, lysed and ChIP was performed with antibodies recognizing AR (SCBT, sc-7305; RRID:AB_626671), GCN5 (SCBT, SC-365321; RRID:AB_10846182), H3K14ac (CST, 7627S; RRID:AB_10839410), H3K27me3 (CST, 9733S; RRID:AB_2616029), NCOR1 (Bethyl Laboratories, Cat#A301-145A; RRID:AB_873085), EZH2 (CST, 5246S; RRID:AB_10694683), or IgG (Abcam, ab2410; RRID:AB_303052), immobilized on Protein A magnetic beads (Biorad, Cat# 161-4013). The complexes were washed with ChIP buffers, eluted with elution buffer (Active Motif, Cat#53008), and subjected to reverse crosslinking, followed by RNase and proteinase K treatments. A part of soluble chromatin was processed separately at the same time and designated as input DNA. Treated ChIP DNA and input DNAs were purified using PCR-DNA purification columns (Qiagen, Cat#28106). The amount of immunoprecipitated DNA was determined by real-time PCR.