Mononuclear Cell Profiling by Flow

Absolute numbers of mononuclear cell populations in peripheral blood were determined by flow cytometry as described (43 (link)), with the addition of anti-NK cell (CD159) antibody (NKG2A; clone Z199, Beckman Coulter).

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Zhang H., Guo H., Lu L., Zahorchak A.F., Wiseman R.W., Raimondi G., Cooper D.K., Ezzelarab M.B, & Thomson A.W. (2015). Sequential monitoring and stability of ex vivo-expanded autologous and non-autologous regulatory T cells following infusion in non-human primates. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 15(5), 1253-1266.

Publication 2015

NKG2A; clone Z199

Antibody Blood Clone Flow cytometry Nk cell Populations

Corresponding Organization : University of Pittsburgh

Other organizations : University of Wisconsin–Madison

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Variable analysis

independent variables

Absolute numbers of mononuclear cell populations in peripheral blood

dependent variables

Absolute numbers of mononuclear cell populations in peripheral blood

control variables

Flow cytometry analysis as described in the referenced study (43)

controls

Positive control: None specified
Negative control: None specified

Annotations

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