C. zedoaria essential oil with different concentrations (75, 150, and 300 µL/mL) (0.2 ml) were added to each test tube containing 0.2 ml of protoscoleces and then kept for 5, 10, 20 and 30 min at 37°C. After the incubation time, the supernatant was removed and 50 µL of 0.1% eosin stain (Sigma-Aldrich, St. Louis, MO, USA) was added to the protoscoleces. After 10 min of incubation, the protoscoleces were smeared on a glass slide, covered with a coverslip, and tested under a light microscope. The results were reported in the percentage of dead and live protoscoleces with the counting of 300 protoscoleces [14 (link)].
Protocol detail
Evaluating Antiparasitic Effect of Zedoaria Essential Oil
C. zedoaria essential oil with different concentrations (75, 150, and 300 µL/mL) (0.2 ml) were added to each test tube containing 0.2 ml of protoscoleces and then kept for 5, 10, 20 and 30 min at 37°C. After the incubation time, the supernatant was removed and 50 µL of 0.1% eosin stain (Sigma-Aldrich, St. Louis, MO, USA) was added to the protoscoleces. After 10 min of incubation, the protoscoleces were smeared on a glass slide, covered with a coverslip, and tested under a light microscope. The results were reported in the percentage of dead and live protoscoleces with the counting of 300 protoscoleces [14 (link)].
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Variable analysis
independent variables
- Concentration of
C. zedoaria essential oil (75, 150, and 300 µL/mL) - Incubation time (5, 10, 20, and 30 min)
- Percentage of dead and live protoscoleces
- Volume of protoscoleces (0.2 ml)
- Temperature (37°C)
- Staining method (0.1% eosin stain)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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